PhD Candidate and Proteomics Technician

Kelly Hodge

k.z.hodge@dundee.ac.uk
Kelly Hodge
Scotland
Kelly Hodge

Project Description


I a Wellcome Trust funded part-time PhD student in Angus’ lab where my project utilises mass spectrometry and laser micro-dissection to perform a comparative, quantitative analysis of the proteomic differences in the hypoxic regions of solid tumours compared to normoxic regions and healthy tissue, to gain a comprehensive picture of what is happening under each of these conditions and its potential effect on tumour progression. To simplify my project, in practice will be split into two parts; first, development and optimisation of laser micro-dissected (LCM) frozen tissue mass spectrometry analysis, then second, application of the optimised workflow on hypoxic tumours.

I am also part of the GRE Proteomics support team where I teach new users of mass spectrometry how to get the best from their experiments and data. Together with Sara ten Have, I advise the best experimental design, how to prepare the samples, the type of mass spectrometry we do in the college and, finally, how to properly analyse the data.



Figure
Proteomics work flow
PepTracker
PepTracker provides management and mining capabilities for data generated during mass spectrometry studies.
Data Shop
Visualisation and statistical analysis tool for quantitative datasets
Proteomics Support
The Proteomics Support team have created a website that provides useful resources for proteomics studies.
Encyclopedia of Proteome Dynamics
A collection of multi-dimensional proteome properties from large-scale mass spectrometry experiments
Cell Biologist's Guide
The Cell Biologist's Guide to Proteomics provides information about mass spectrometry and experimentation.