Postdoctoral Researcher

Dr Kayo Ono nee Yamada

k.ono@dundee.ac.uk
Kayo Ono
Japan
Kayo Ono

Project Description


We are studying protein dynamics in nucleoli, chromosome, nucleoplasm, and cytoplasm of various cells during tumourogenesis using proteomics approach, and characterising selected novel tumour associated proteins from our analysis (Ref. 1 & 4). Our recent discovery is FMN2, which is a novel regulator of the cyclin-dependent kinase inhibitor p21 (Patent 1, Ref. 6 & 8). FMN2 is stabilising p21 in protein level p53 independently, and regulated by induction of p14ARF and by multiple other stress responses, including DNA damage and hypoxia, which have in common activation of cell cycle arrest. We are investigating detail mechanisms of stabilization of p21 via FMN2 at the various stresses as we described above. I'm also contributing to the small nucleolar RNA (snoRNA) modulator of gene expression (snoMEN) vector development and filing some patents from both studies (Patent 2, Ref. 2, 3, 5, & 7).


Japanese

私たちは様々な細胞のガン化に伴うタンパクのダイナミクスを、核小体、核質、クロモゾーム、細胞質などの分核に分けて解析しており、この解析から得られた情報を元に、新規のタンパク質の機能を解析しています (Ref. 1 & 4)。最近の発見では、FMN2というcyclin-dependent kinase inhibitor p21を制御する新規のタンパクを報告しています (Patent 1, Ref. 6 & 8)。FMN2はp53非依存的にp21のタンパクの安定性に関わっており、p14ARFによる癌抑制刺激だけでなく、DNAダメージや、ハイポキシアなどの一般的に細胞周期停止につながるとされる複数のストレス刺激に応答します。私たちは現在これらの刺激下において、FMN2がどのようにp21を安定化させているkについての詳細なメカニズムについて解析を進めいています。 また、私は核小体のsmall nucleolar RNA (snoRNA)を使った、snoRNA modulator of gene expression (snoMEN) ベクターの開発にも携わっています(Patent 2, Ref. 2, 3, 5, & 7)。


Patents

1. Yamada, K., Ono, M., Rocha, S., Lamond, A.I. (2010). Diagnosis and treatment of cell proliferation and differentiation disorders based on the fmn2 gene. International patent specification, WO/2010/103284.

2. Ono, M., Yamada, K., and Lamond, A.I., (2013). Targeting of miRNA precursors. WO/2013/054113.


References

1. van Kriegsheim A, Baiocchi D, Birtwistle M, Sumpton D, Bienvenut W, Morrice N, Yamada K, Lamond AI, Kalna G, Orton R, Gilbert D, Kolch W (2009) Cell fate decisions are specified by the dynamic ERK interactome. Nat Cell Biol 11: 1458-1464.

2. Ono M, Yamada K, Avolio F, Scott MS, van Koningsbruggen S, et al. (2010) Analysis of human small nucleolar RNAs (snoRNA) and the development of snoRNA modulator of gene expression vectors. Mol Biol Cell 21: 1569-1584.

3. Ono M*, Scott MS*, Yamada K, Avolio F, Barton GJ, et al. (2011) Identification of human miRNA precursors that resemble box C/D snoRNAs. Nucleic Acids Res 39: 3879-3891.

4. Kubota T, Hiraga S, Yamada K, Lamond AI, Donaldson AD. (2011) Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint. Mol Cell Proteomics 10: M110.005561

5. Scott MS*, Ono M*, Yamada K, Endo A, Barton GJ, et al. (2011) Human box C/D snoRNA processing conservation across multiple cell types. Nucleic Acids Res.

6. Yamada K*, Ono M*, Perkins ND, Rocha S, Lamond AI (2013) Identification and functional characterization of FMN2, a regulator of the cyclin-dependent kinase inhibitor p21. Molecular cell 49: 922-933. [awarded the Haward Elder Prize 2013]

7. Ono M*, Yamada K*, Endo A*, Avolio F, Lamond AI (2013) Analysis of human protein replacement stable cell lines established using snoMEN-PR vector. PloS one 8: e62305. [Awarded the best poster prize at GRE symposium 2013]

8. Yamada K, Ono M, Bensaddek D, Lamond AI, Rocha S (2013) FMN2 is a novel regulator of the cyclin-dependent kinase inhibitor p21. Cell cycle 12: 2348-2354.

* Joint authorship.

Figure
Figure 1.Proposed mode of action for FMN2.
PepTracker
PepTracker provides management and mining capabilities for data generated during mass spectrometry studies.
Data Shop
Visualisation and statistical analysis tool for quantitative datasets
Proteomics Support
The Proteomics Support team have created a website that provides useful resources for proteomics studies.
Encyclopedia of Proteome Dynamics
A collection of multi-dimensional proteome properties from large-scale mass spectrometry experiments
Cell Biologist's Guide
The Cell Biologist's Guide to Proteomics provides information about mass spectrometry and experimentation.