I did my PhD in Virology at Ecole Normale Supérieure de Lyon in France where I studied the replication of the small, non-pathogenic helper virus-dependent human Parvovirus "Adeno-Associated Virus" (AAV) under the supervision of Dr. Anna Salvetti. As part of my main project, we collaborated with a proteomics lab in Grenoble to identify by a TAP-tag/LC-MS/MS strategy host and viral proteins which locate into viral replication compartments during Herpes Simplex Virus 1-mediated AAV replication. This first proteomics experiment sparkled my interest for proteomics in general and mass spectrometry in particular. I now work on methods for subcellular fractionation and determination of the localisation of proteins within cells, which I am applying – together with other proteomics workflows – to the study of a wholly different virus: the dreadful Foot and Mouth Disease Virus of the 2001 UK epidemic fame.
B0003384 Credit David Stuart, Uni. of Oxford, Wellcome Images
Structure of foot-and-mouth disease virus
The three-dimensional X-ray crystallographic
structure of foot-and-mouth disease virus at 2.9
Image created by Ravindra Acharya, Elizabeth Fry,
David Stuart, Graham Fox, David Rowlands and Fred
Wellcome Image Award winner 2003
Collection: Wellcome Images
Library reference no.: Contributor Reference GOOD COVER.J
Copyrighted work available under Creative Commons by-nc-nd 2.0 UK: England & Wales, see http://wellcomeimages.org/indexplus/page/Prices.html
PepTracker provides management and mining capabilities for data generated during mass spectrometry studies.
Visualisation and statistical analysis tool for quantitative datasets
The Proteomics Support team have created a website that provides useful resources for proteomics studies.
A collection of multi-dimensional proteome properties from large-scale mass spectrometry experiments
The Cell Biologist's Guide to Proteomics provides information about mass spectrometry and experimentation.